Collagen Extra® Peptan® SR marine 50ml
Anti-ageing formula with clinically proven collagen peptides and hyaluronic acid
3-step skin regeneration collagen peptides:
- Stimulates fibroblast proliferation
- Boosts collagen synthesis
- Protects against oxidative stress
Collagen Extra® anti-ageing formula with collagen Peptan® SR marine.
This formula features Peptan® SR marine collagen peptides to provide the amino acids required for the correct functioning of skin cells.
- It boosts fibroblast proliferation and favours the synthesis of the major structural collagen molecule of the dermis, thus preserving its structural organization.
- Protects against free radicals, limiting their ageing effects
Peptan® SR marine activates collagen synthesis by fibroblasts. It restores the three dimensional architecture of the dermis, increasing the volume of the tissue providing it with cohesion and tonicity. It affects the extracellular matrix of the dermis in order to counteract the loss of firmness which occurs over time, and eventually leads to wrinkles. Peptan® SR marine provides protection against agents generating free radicals.
EFFICACY TESTS
STIMULATION OF FIBROBLAST
PROLIFERATION XTT cell proliferation test Cultured human skin fibroblasts were treated after 24 hours with Peptan® SR marine at a concentration of 0.1 mg/ml. After incubation for 24 h, XTT cell proliferation test was used to assess the viability and proliferation of the fibroblasts. The absorbance at 450 nm is determined by spectrophotometry and the results are expressed as a percentage of the value for untreated controls. The incubation of 0.1 mg/ml Peptan® SR with cultured fibroblasts for 24 hours induced a significant increase in fibroblast proliferation to 18% higher than that of an untreated control (p=0.016).
STIMULATION OF COLLAGEN SYNTHESIS
IN VITRO TEST VS BSA CONTROL Human dermal fibroblasts were cultured and, 24 hours later, 0.01 mg/ml Peptan® SR marine was added to the culture medium. Asacontrol, fibroblasts were incubated with 0.01 mg/ml BSA. Two days later, collagen I in fibroblasts was quantified by staining with Syrius Red by a fluorimetric method. The treatment of fibroblasts with 0.01 mg/ml Peptan® SR for 2 days induced a significant increase in collagen synthesis to 22.5% higher than that of the BSA-treated control (p<0.05). Figure 2: Stimulation of collagen synthesis by fibroblasts induced by Peptan® SR marine (0.01 mg/ml), as shown by comparison with Control at 2 days.
EFFECT OF PEPTAN® SR marine ON THE ORGANIZATION AND DENSITY OF FIBROBLASTS Figure 3: Fibroblasts incubated for 7 days with 0.01 mg/ml BSA (left) and 0.01 mg/ ml Peptan® SR marine (right), after staining with Sirius Red.
PROTECTION AGAINST
OXIDATIVE STRESS ORAC TEST IN VITRO Decoloration of beta carotene was assessed in the presence and absence of 5 mg/ml Peptan® SR marine. The positive control used was butylhydroxyanisol (BHA) at a concentration of 2.5 mg/ml. Spectrofluorimetry was used and the results are expressed with respect to the protection against
oxidation conferred by the reference antioxidant (BHA) per gram of product tested. The antioxidant activity of Peptan® SR (5 mg/ml) was about 62% of that of BHA.